The Center for Nanobiophotonics And Laser Microspectroscopy develops an interdisciplinary research focused on the fabrication and biofunctionalization of noble metal, semiconductor and polymer nanoparticles and hybrid nanostructures that perform novel function in nanophotonics and plasmonics with the aim of enabling novel spectroscopic and plasmonic-oriented applications. Noble-metal nanostructures (Ag, Au) exhibit strong interaction with the visible light due to the excitation of collective electron oscillations (localized surface plasmons) and, on the other hand, can bind specifically to many biological entities (biomolecules, proteins, cells, bacteria).
Currently, we study the interactions between nanostructures and biological entities with standard optical spectroscopy (uv-visible, Raman, fluorescence) and advanced methods based on scanning confocal Raman microscopy, surface-enhanced Raman spectroscopy (SERS), surface-enhanced IR absorption (SEIRA), confocal reflectivity and fluorescence, localized surface plasmon resonances (LSPR), dark-field microscopy in combination with Atomic Force Microscoopy AFM.
We offer technical characterization of materials by Raman, fluorescence, and reflectivity with high performance instrumentation based on confocal Raman microscope (Alpha 300, three excitation wavelengths at 532 nm, 633 nm and 785 nm, detection between 100 - 3500 wavenumbers and lateral resolution better than ~ 250 nm) which is integrated with an atomic force microscope (AFM) of high spatial resolution and different operation modes. For more details concerning our infrastructure visit our Laboratory section. We are able to characterize, identify and image non-destructively chemical components and their molecular structure existing in heterogeneous materials, thin inorganic films, polymers, semiconductors, glasses, etc. in nanotechnology, life science, geology, pharmaceutical and food industry.
Additionally, we provide a large variety of nanostructured substrates (highly organized, regular arrays of noble-metal nanoparticles and films) with distinct optical properties and bio-chemical functionalities to operate as optical probe in bio- and chemical- sensing platform in the field of molecular biology, medicine and environment monitoring.